|
KMID : 1094720090140030302
|
|
Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 3 p.302 ~ p.306
|
|
|
Development of Fermentation Process for PLA-degrading Enzyme Production by a New Thermophilic Actinomadura sp. T16-1
|
|
Sukhumaporn Sukkhum
Shinji Tokuyama
Vichien Kitpreechavanich
|
|
|
Abstract
|
|
|
|
The fermentation process for a poly (L-lactide) (PLA)-degrading enzyme production by a newly isolate of thermophilic PLA-degrading Actinomadura sp. T16-1 was investigated. The strain produced 33.9 U/mL of enzyme activity after cultivation at 50꼆C under shaking of 150 rpm for 96 h in a medium consisting of (w/v) 0.05% PLA film, 0.2% gelatin, 0.4% (NH4)2SO4, 0.4% K2HPO4, 0.2 % KH2PO4, and 0.02% MgSO4 ? 7H2O. The optimal concentration of PLA film and gelatin obtained by response surface methodology (RSM) for the highest production of PLA-degrading enzyme was 0.035% (w/v) and 0.238% (w/v), respectively. Under these conditions, the model predicted 40.4 U/mL of PLA-degrading activity and the verification of the optimization showed 44.6 U/mL of PLA-degrading enzymatic activity in the flasks experiment. The maximum PLA-degrading activity reached 150 U/mL within 72 h cultivation in the 3-L airlift fermenter.
|
|
|
KEYWORD
|
|
|
Actinomadura sp., optimization, PLA-degrading enzyme, response surface methodology
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|